When the dialog opens to select an FCS file, change the Files of type field to BD Accuri C6 files (.c6,.cfl). Select the BD Accuri software file that contains the data you want to view. FCS Express now populates the dialog with all of the Sample Wells that are contained within the BD Accuri software file. Imported Gated FCS from FlowJo to Cytobank - FCS2.0? Has anyone gated in Flowjo (I gate all the way down to specific T and B cell subsets), exported the FCS files, imported into Cytobank and tried to run ViSNE?
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commentedMight 17, 2016
Hello everyone, I was attempting to use one flowJo workspace.XML file to utilize it to several.FCS files (that were obtained with the exact same variables). Nevertheless, Ur (I'michael making use of openCyto deal) seems to need even more a individual work area for each.FCS file to perform the gating. So, I has been asking yourself wehn R says the workspace, what parameters precisely will it get for gating and can we feasible make use of the exact same work area to gate several.fcs data that had been acaquired with specific same guidelines? Thank you in advance, |
commentedMight 17, 2016
If your FCS documents have the same parameters and you would like to just apply the exact same static entrance to all the files: If you are usually speaking about extracting the gating scheme from the workspace and produce the csv gating template with the appropriateautomated gating formula automatically established for each gate/inhabitants, then it is certainly various from what you previously pointed out, and it is not yet obtainable. |
mentionedMay 18, 2016
Say thanks to you. Yes, I do really mean that - '. removing the gating scheme from the workspace and produce the csv gating template with the appropriate automated gating criteria automatically driven for each gate/people.' However, by 'merely utilize the samestatic gatesto all the data files', you indicate by static entrances that the area of the gates will be the exact same in all the.fcs documents where i would apply the gating derived from 1 wflowJo workspace? |
commentedMight 18, 2016
@anaraserikbek This can be on our radar. Not really implemented however. |
mentionedMay 18, 2016
@anaraserikbek , yes, that's what I intended. |
referenced this issueJun 28, 2017
ClosedUsing flowJo workspace to gate FCS data files#152
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